Expression of Annexin-1 in patients with endometriosis / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 927-931, 2008.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-258564
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Annexin-1 was identified as an endometriosis-related protein by comparative proteomics in previous study. As an endogenous anti-inflammatory mediator, Annexin-1 has been shown to regulate the immune response, cell proliferation and apoptosis. To investigate whether Annexin-1 is involved in the pathogenesis of endometriosis, we examined the expression of Annexin-1 in eutopic endometrium of women with or without endometriosis, and detected its expression in peritoneal fluids of those with endometriosis.</p><p><b>METHODS</b>Eutopic endometrium samples from twenty-five women with endometriosis and those from sixteen age-matched women without endometriosis were collected. Peritoneal fluids were obtained from ten patients with endometriosis. The expression of Annexin-1 protein in eutopic endometrium was detected by immunohistochemistry and Western blotting, and mRNA detected by real-time PCR. Annexin-1 protein in the peritoneal fluids was detected by Western blotting.</p><p><b>RESULTS</b>Annexin-1 mRNA and protein were overexpressed in eutopic endometrium of endometriosis without significant differences between the proliferative and secretory phase. Immunohistochemistry showed that Annexin-1 protein was expressed mainly in endometrial glandular cells throughout the menstrual cycle. Annexin-1 protein was detected in the peritoneal fluids of all the ten patients with endometriosis.</p><p><b>CONCLUSIONS</b>Annexin-1 is overexpressed in eutopic endometrium and presents in the peritoneal fluids of patients with endometriosis, and may play a role in the pathogenesis of endometriosis.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Ascitic Fluid
/
RNA, Messenger
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Immunohistochemistry
/
Gene Expression
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Blotting, Western
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Annexin A1
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Reverse Transcriptase Polymerase Chain Reaction
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Endometriosis
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Genetics
Limits:
Adult
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Female
/
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2008
Document type:
Article