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Comparison of the transcriptional levels of mannitol PTS operon between epidemic and non-epidemic strains of Vibrio cholerae / 中国医学科学院学报
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-259075
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare the transcription difference of the mannitol PTS genes between epidemic and non-epidemic strains of Vibrio cholerae El Tor in mannitol ferment tests.</p><p><b>METHODS</b>Growth curves of 10 epidemic strains (slow-ferment) and 10 non-epidemic strains (rapid-ferment) of Vibrio cholerae were detected in the process of fermentation test, and the transcriptional level of mannitol PTS operon of these strains were determined with quantitative reverse-transcriptional PCR.</p><p><b>RESULTS</b>After 4 hours of test, the non-epidemic strains became positive and the average growth density of the non-epidemic strains was higher than that of the epidemic strains; however, some were still lower than the epidemic strains. In contrast, at the eighth hour of test, when epidemic strains got positive, they showed higher average growth density. Compared to the epidemic strains, the transcription of mannitol PTS genes of the non-epidemic strains were much more active at the 1st and 2nd hour and were lower at the 4th and 8th hour.</p><p><b>CONCLUSIONS</b>The difference of mannitol PTS operon transcription level should be an important feature to identify the epidemic and non-epidemic strains of Vibrio cholerae, which directly influences the mannitol fermentation rate during the test. The growth rate is not a key factor that affect such difference.</p>
Subject(s)
Full text: Available Health context: Neglected Diseases Health problem: Cholera / Neglected Diseases Database: WPRIM (Western Pacific) Main subject: Operon / Phosphoenolpyruvate / Sugar Alcohol Dehydrogenases / Bacterial Proteins / Transcription, Genetic / Vibrio cholerae / Gene Expression Regulation, Bacterial / Classification / Reverse Transcriptase Polymerase Chain Reaction / Genetics Type of study: Prognostic study Language: Chinese Journal: Acta Academiae Medicinae Sinicae Year: 2009 Document type: Article
Full text: Available Health context: Neglected Diseases Health problem: Cholera / Neglected Diseases Database: WPRIM (Western Pacific) Main subject: Operon / Phosphoenolpyruvate / Sugar Alcohol Dehydrogenases / Bacterial Proteins / Transcription, Genetic / Vibrio cholerae / Gene Expression Regulation, Bacterial / Classification / Reverse Transcriptase Polymerase Chain Reaction / Genetics Type of study: Prognostic study Language: Chinese Journal: Acta Academiae Medicinae Sinicae Year: 2009 Document type: Article
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