Relationship of the expression of cyclin D1 and P21WAF1 with the sensitivity to chemotherapeutic drugs on gastric cancers / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 615-617, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-259354
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between the sensitivity to chemotherapeutic drugs and the expression of cyclin D1 and P21WAF1 in gastric carcinoma.</p><p><b>METHODS</b>The sensitivity of 80 samples of gastric cancer cells to HCPT, CDDP, 5-FU, ADM and MMC was evaluated with MTT assay. The protein expression of cyclin D1 and P21WAF1 was detected by immunohistochemistry.</p><p><b>RESULTS</b>The sensitivity of gastric carcinoma cells to chemotherapeutic drugs was different. Inhibitory rates of tumor cells exposed to 5-FU, MMC and DDP were significantly higher than those exposed to ADM and HCPT (P <0.05). Positive expression rates of cyclin D1 and P21WAF1 in gastric cancer were 70.0% and 47.5% respectively. Cyclin D1 positive cells showed more sensitive to 5-FU and HCPT than cyclin D1 negative cells. P21WAF1 negative cells showed more sensitive to MMC, 5-FU and DDP than P21WAF1 positive cells.</p><p><b>CONCLUSION</b>Expression of cyclin D1 and P21WAF1 is related with drug-resistance of tumor. Examination of cyclinD1 and P21WAF1 would have reference value in selection of chemotherapeutic drugs.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Stomach Neoplasms
/
Drug Screening Assays, Antitumor
/
Cyclin D1
/
Drug Therapy
/
Cyclin-Dependent Kinase Inhibitor p21
/
Metabolism
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2009
Document type:
Article