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Effect of siRNA targeting c-Myc and VEGF on human colorectal cancer cells / 中华胃肠外科杂志
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-259379
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biological behavioral effects of specific siRNA expression plasmids targeted against c-Myc and vascular endothelial growth factor (VEGF) on human colorectal cancer cell line Volo.</p><p><b>METHODS</b>The expression plasmids with small interfering RNA (siRNA) aiming at c-Myc and VEGF were designed and constructed respectively, then transfected into Volo cells by eukaryocyte transfection technique. The protein expressions of c-Myc and VEGF were detected by Western blotting. Cellular proliferation, apoptosis, cycle distribution and invasion character were analyzed by tetrazolium bromide colorimetry (MTT), flow cytometry (FCM), TUNEL assay and matrigel invasion assay respectively.</p><p><b>RESULTS</b>Enzymatic digestion and DNA sequencing confirmed that the c-Myc and VEGF specific siRNA expression plasmids were constructed successfully. After plasmids were transfected into cells, the protein expressions of c-Myc and VEGF were significantly down-regulated respectively as compared with control group (P<0.01). The cellular proliferation inhibitory rates in c-Myc siRNA group, VEGF siRNA group and c-Myc+VEGF group were (59.20+/-5.05)%, (32.31+/-3.48)% and (75.81+/-7.89)% respectively, which were higher than that in control group [(6.80+/-1.45)%] (all P<0.05). The cell apoptosis rate in above 3 groups were (40.50+/-4.37)%, (21.30+/-2.98)% and (62.59+/-9.66)% respectively, which were higher than that in control group [(2.90+/-0.36)%] (all P<0.05). The cell invasion rates in VEGF siRNA group and c-Myc+VEGF siRNA group were (7.34+/-3.65)% and (2.80+/-1.02)%, which were lower than that in control group [(18.57+/-7.46)%] (P<0.05). The effect of c-Myc+VEGF siRNA group was greater.</p><p><b>CONCLUSIONS</b>The specific siRNA efficiently silences the expression of c-Myc and VEGF, subsequently, suppresses the cell proliferation, triggers the cell apoptosis and inhibits the cell invasiveness in these transfected colorectal cancer Volo cells. In addition, the synergism of siRNA-c-Myc and siRNA-VEGF in transfected cells can be found.</p>
Subject(s)
Full text: Available Health context: SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases Health problem: Colon and Rectum Cancers Database: WPRIM (Western Pacific) Main subject: Pathology / Colorectal Neoplasms / Genetic Therapy / Genes, myc / Apoptosis / RNA, Small Interfering / Cell Line, Tumor / Vascular Endothelial Growth Factor A / Cell Proliferation / Genetics Limits: Humans Language: Chinese Journal: Chinese Journal of Gastrointestinal Surgery Year: 2009 Document type: Article
Full text: Available Health context: SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases Health problem: Colon and Rectum Cancers Database: WPRIM (Western Pacific) Main subject: Pathology / Colorectal Neoplasms / Genetic Therapy / Genes, myc / Apoptosis / RNA, Small Interfering / Cell Line, Tumor / Vascular Endothelial Growth Factor A / Cell Proliferation / Genetics Limits: Humans Language: Chinese Journal: Chinese Journal of Gastrointestinal Surgery Year: 2009 Document type: Article
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