Analysis of methylation and loss of heterozygosity of RUNX3 gene in hepatocellular carcinoma and its clinical significance / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 227-230, 2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-260054
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>In order to elucidate role of RUNX3 gene in hepatocarcinogenesis, we detected genetic and epigenetic alteration of RUNX3 gene in hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>PCR-SSCP, analysis of loss of heterozygosity (LOH), sequencing and methylation-specific PCR (MSP) were used to detect mutation, LOH and DNA methylation of RUNX3 gene in 90 HCCs.</p><p><b>RESULTS</b>No mutation was found, but three single-nucleotide polymorphisms (SNP) were found and distributed over exon1 and exon4. 30.6% (11/36) of cases showed LOH; 54.4% (49/90) of cases was in hypermethylation. There is a significant correlation between LOH and major portal vein invasive or micro vessel invasion or intrahepatic metastasis.</p><p><b>CONCLUSION</b>High frequent hypermethylation and LOH of RUNX3 gene were found in HCC. Aberrant RUNX3 gene may play an important role in the development of HCC.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Transcription Factors
/
Carcinoma, Hepatocellular
/
DNA Methylation
/
Loss of Heterozygosity
/
DNA-Binding Proteins
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Core Binding Factor Alpha 3 Subunit
/
Genetics
/
Liver Neoplasms
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2004
Document type:
Article