Simultaneous determination of plantainoside D and verbascoside from stem of Chirita longgangensis var. hongyao by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3188-3191, 2010.
Article
in Zh
| WPRIM
| ID: wpr-260724
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a RP-HPLC method for simultaneous determination of phenylethanoid glycosides plantainoside D and verbascoside in Chirita longgangensis var. hongyao.</p><p><b>METHOD</b>The analysis was performed on a Agilent C18 column (4.6 mm x 250 mm, 5 microm) with CH3CN-1% HAc (16:84)as mobile phase at a flow rate of 1.0 mL x min(-1), and at a column temperature of 30 degrees C. The detection wave length was 332 nm.</p><p><b>RESULT</b>The linear ranges of calibration of plantainoside D and verbascoside were 3.125-100.00 mg x L(-1) (r = 0.9998) and 25.00-500.0 mg x L(-1) (r = 0.9998). The average recoveries were 101.3% and 100.8% with RSD of 2.6% and 2.2% (n=9), respectively.</p><p><b>CONCLUSION</b>The method is simple, accurate, reliable and can be used for the quality evaluation of C. longgangensis var. hongyao and its preparation.</p>
Full text:
1
Database:
WPRIM
Main subject:
Phenols
/
Plant Extracts
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Plant Stems
/
Magnoliopsida
/
Coumaric Acids
/
Disaccharides
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Chromatography, Reverse-Phase
/
Glucosides
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Document type:
Article