Protection and mechanism of total flavone of Hippophae rhamnoides on vascular endothelial cells / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 355-358, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-260989
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the protection of total flavones of Hippophae Rhamnoides (TFH) on vascular endothelial cells (VECs).</p><p><b>METHODS</b>Human umbilical VECs (ECV304) were used. The vascular endothelial injured cell model was prepared using hydrogen dioxide (H2O2). The cell apoptosis rate and changes of mean fluorescence intensity were detected using flow cytometry (FCM). The Caspase-3 activity in VECs was detected by Western blot.</p><p><b>RESULTS</b>VEC apoptosis was induced by 200 micromol/L H2O2. TFH in different concentrations (400, 200, and 100 microg/mL) could significantly lower the cell apoptosis rate induced by H2O2 respectively (all P < 0.05), and obviously inhibit Caspase-3 activities (all P < 0.01).</p><p><b>CONCLUSIONS</b>TFH could fight against H2O2 injured VECs apoptosis. Lowering the Caspase-3 expression was one of its mechanisms in protecting VECs.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cell Survival
/
Cells, Cultured
/
Apoptosis
/
Protective Agents
/
Hippophae
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Endothelial Cells
/
Flavones
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Caspase 3
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2011
Document type:
Article