Airway inflammation induced by Poly(I:C) stimulation in the late stage of respiratory syncytial virus infection in mice and its mechanism / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 455-459, 2016.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-261210
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the pathogenic mechanisms of airway inflammation and recurrent wheezing induced by recurrent respiratory virus infection after respiratory syncytial virus (RSV) infection.</p><p><b>METHODS</b>Sixty-four female BALB/c mice (aged 6-8 weeks) were randomly divided into four groups control, RSV, Poly(IC), and RSV+Poly(IC) (n=16 each). The bronchoalveolar lavage fluid (BALF) was collected on the 3rd day after Poly(IC) administration, and the total cell number and differential counts in BALF were determined. Hematoxylin-eosin staining was used to observe pulmonary pathological changes. The airway responsiveness was detected. ELISA was used to measure the levels of interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-13 (IL-13), matrix metallopeptidase-9 (MMP-9), and tissue inhibitor of metalloproteinase-1 (TIMP-1) in BALF.</p><p><b>RESULTS</b>Compared with the other three groups, the RSV+Poly(IC) group had significant increases in the total number of inflammatory infiltrating cells in the airway, airway responsiveness, and MMP-9 level in BALF (P<0.05). The RSV+Poly(IC) group showed more severe pulmonary tissue injuries compared with the control and RSV groups (P<0.01). Compared with the RSV group, the RSV+Poly(IC) group showed significant reductions in the levels of IL-4 and TIMP-1 in BALF (P<0.01).</p><p><b>CONCLUSIONS</b>Viral re-infection in the late stage of RSV infection may cause an imbalance of MMP-9/TIMP-1 expression and thus contribute to aggravated airway inflammation.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
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Asthma
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Bronchoalveolar Lavage Fluid
/
Chemistry
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Poly I-C
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Respiratory Syncytial Virus Infections
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Tissue Inhibitor of Metalloproteinase-1
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Matrix Metalloproteinase 9
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Lung
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2016
Document type:
Article