Ex vivo expansion of CD34(+) CD59(+) cells from bone marrow of paroxysmal nocturnal hemoglobinuria patients / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the separation, purification and ex vivo expansion of CD(34)(+) CD(59)(+) cells from patients with paroxysmal nocturnal hemoglobinuria (PNH), and explore the new treatment for the PNH patients.</p><p><b>METHODS</b>CD(34)(+) CD(59)(+) cells were selected from the bone marrow mononuclear cells of PNH patients by means of immunomagnetic microbead-flow cytometry two step sorting method, followed by ex vivo expansion of the cells with combination of hematopoietic factors for two weeks.</p><p><b>RESULTS</b>The best combination for the ex vivo expansion was SCF + IL-3 + IL-6 + FL + Tpo + Epo, and the seventh day was the most suitable time for the best harvest when the CD(34)(+) CD(59)(+) cells were 22.42 +/- 3.73 fold expanded. After ex vivo expansion, the cells remained CD(59) positive and potent capacity of colony formation, but their potentialities to multilineage differentiation were decreased.</p><p><b>CONCLUSION</b>The present study shows that ex vivo expansion of CD(34)(+) CD(59)(+) cells from PNH patients might promise the possibility of performing ABMT or APBSCT clinincally for the patients.</p>