Antiproliferation of cardamonin associated with mRNA expression of mTOR, Raptor and Rictor / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2318-2323, 2010.
Article
in Zh
| WPRIM
| ID: wpr-262167
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the antiproliferation effect of cardamonin (CAR) and its possible mechanisms on human umbilical artery smooth muscle cells (HUASMCs) cultured in the mimicking insulin resistance (IR) medium.</p><p><b>METHOD</b>Proliferation of HUASMCs was assayed by MTT method. The mRNA expression of mTOR, Raptor and Rictor was detected by a real-time PCR. The expression content was calculated by Livak method using internal control of beta-actin.</p><p><b>RESULT</b>The proliferation of HUASMCs cultured in the mimicking IR medium was significantly increased. Both in normal and mimic IR culture medium, cells proliferation was inhibited by CAR (1 x 10(-5), 1 x 10(-4) mol x L(-1)). Pretreated with PD98059 and LY294002, cell proliferation induced by phosphatidic acid (PA) was inhibited, and the mRNA expression of mTOR, Raptor and Rictor was significantly decreased by CAR in the mimic IR medium.</p><p><b>CONCLUSION</b>It is implicated that antiproliferation of CAR is involved in mRNA expression decrease of mTOR and its relative protein Raptor and Rictor.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
RNA, Messenger
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Carrier Proteins
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Cells, Cultured
/
Gene Expression Regulation
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Cell Biology
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Myocytes, Smooth Muscle
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Chalcones
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Adaptor Proteins, Signal Transducing
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Cell Proliferation
Limits:
Humans
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Document type:
Article