Inhibition of mitochondria responsible for the anti-apoptotic effects of melatonin during ischemia-reperfusion / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 142-147, 2006.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-263213
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate a possible mechanism responsible for anti-apoptotic effects of melatonin and provide theoretical evidences for clinical therapy.</p><p><b>METHODS</b>Ischemia-reperfusion mediated neuronal cell injury model was constructed in cerebellar granule neurons (CGNs) by deprivation of glucose, serum and oxygen in media. After ischemia, melatonin was added to the test groups to reach differential concentration during reperfusion. DNA fragmentation, mitochondrial transmembrane potential, mitochondrial cytochrome c release and caspase-3 activity were observed after subjecting cerebellar granule neurons to oxygen-glucose deprivation (OGD).</p><p><b>RESULTS</b>The results showed that OGD induced typical cell apoptosis change, DNA ladder and apoptosis-related alterations in mitochondrial functions including depression of mitochondrial transmembrane potential (its maximal protection ratio was 73.26%) and release of cytochrome c (its maximal inhibition ratio was 42.52%) and the subsequent activation of caspase-3 (its maximal protection ratio was 59.32%) in cytoplasm. Melatonin reduced DNA damage and inhibited release of mitochondrial cytochrome c and activation of caspase-3. Melatonin can strongly prevent the OGD-induced loss of the mitochondria membrane potential.</p><p><b>CONCLUSION</b>Our findings suggested that the direct inhibition of mitochondrial pathway might essentially contribute to its anti-apoptotic effects in neuronal ischemia-reperfusion.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Cardiovascular Disease
Database:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Pathology
/
Pharmacology
/
Time Factors
/
Immunoblotting
/
Reperfusion
/
Reperfusion Injury
/
Cerebellum
/
Blotting, Western
/
Rats, Sprague-Dawley
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2006
Document type:
Article