Determination of cirsilineol in Herba Artemisiae scopariae collected in autumn by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a quantitative method for determination of cirsilineol in Herba Artemisiae Scopariae collected in Autumn.</p><p><b>METHOD</b>Sample was extracted with methanol, and cleaned up with polyamide column. ODS column was used with methanol-acetonitrile-0.5% acetic acid solution (241957) as mobile phase. Detection wavelength was 347 nm.</p><p><b>RESULT</b>Cirsilineol in sample solution was well separated. Linearity of cirsilineol was good (r = 0.9998) in range of 0.1-0.8 microgram. The average recovery was 101.3%, RSD of repeatability was 3.04%.</p><p><b>CONCLUSION</b>This method can be used for quality control of Herba Artemisiae Scopariae collected in autumn and its preparations.</p>