In vivo digestive stability of soybean β-conglycinin β-subunit in WZS minipigs / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 85-88, 2015.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-264616
Responsible library:
WPRO
ABSTRACT
By now, the digestive stability experiments provided by most authoritative organizations are in vitro tests. Evaluating the protein digestive stability with in vivo models should be more objective. The present study aimed to verify the in vivo digestibility of soybean β-conglycinin β-subunit in Wuzhishan (WZS) minipigs. Three minipigs were surgically fitted with O-stomach and T-ileum cannulae and fed with soybean meals. According to SDS-PAGE, the 50 kD fraction of soybean β-conglycinin β-subunit persisted in the gastric fluid until 6 h after feeding, which was detected at 3 h and clearly visible at 4-6 h in the intestinal fluid. Western blot with anti-β-conglycinin β-subunit McAb confirmed it.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Swine
/
Swine, Miniature
/
Chemistry
/
Soybean Proteins
/
Protein Subunits
/
Digestion
/
Antigens, Plant
/
Seed Storage Proteins
/
Globulins
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2015
Document type:
Article