Interleukin-1 inhibits Sox9 and collagen type II expression via nuclear factor-kappaB in the cultured human intervertebral disc cells / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 2483-2488, 2009.
Article
in En
| WPRIM
| ID: wpr-266042
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>The most significant biological change in intervertebral disc degeneration is the decrease of chondrocyte specific gene and protein expression of Sox9 and collagen type II. Interleukin-1 (IL-1) is not expressed in the normal intervertebral disc tissue but increases in the degenerated intervertebral disc tissue. This suggests that IL-1 may play a role in regulation of the expression of Sox9 and collagen type II.</p><p><b>METHODS</b>Human intervertebral disc cells were isolated and cultured. Sox9 and collagen type II expression during treatment with IL-1, with or without the nuclear factor-kappaB (NF-kappaB) activity inhibitor curcumin, were detected by using reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, and the activity of the NF-kappaB signaling pathway was detected by the electrophoretic mobility shift assay (EMSA).</p><p><b>RESULTS</b>IL-1 lowered the mRNA level and protein expression of Sox9 and collagen type II in the cultured intervertebral disc cells in a dose dependent manner (P < 0.05), and this effect was attenuated by curcumin. Curcumin alone had no effect on Sox9 and collagen type II expression (P > 0.05). IL-1 at concentrations of 0.1 ng/ml, 1 ng/ml and 10 ng/ml could stimulate the activity of NF-kappaB in the intervertebral disc cells in a dose dependent manner (P < 0.05) that was inhibited by curcumin.</p><p><b>CONCLUSIONS</b>We demonstrated the previously unknown function of IL-1 in inhibiting Sox9 and collagen type II via NF-kappaB in the intervertebral disc cells. This inhibition can be attenuated by curcumin, which is an effective NF-kappaB activity inhibitor.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
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Immunoblotting
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Gene Expression
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Cells, Cultured
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NF-kappa B
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Interleukin-1
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Reverse Transcriptase Polymerase Chain Reaction
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Curcumin
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Cell Biology
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Collagen Type II
Limits:
Adult
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Humans
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Male
Language:
En
Journal:
Chinese Medical Journal
Year:
2009
Document type:
Article