Expression of Na+-H+ exchanger 1 in human gastric carcinoma tissue and its clinical significance / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 604-607, 2010.
Article
in Zh
| WPRIM
| ID: wpr-266302
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the expression of Na+/H+ exchanger 1(NHE1) in human gastric carcinoma tissue and to investigate the association between NHE1 expression and clinicopathological characteristics.</p><p><b>METHODS</b>The expressions of NHE1 mRNA and protein were detected in both gastric carcinoma tissue (n=60) and adjacent gastric mucosa tissue (n=30) by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The association between the expression and the clinicopathological characteristics was analyzed.</p><p><b>RESULTS</b>The relative expression levels of NHE1 mRNA and protein in gastric carcinoma tissue were 0.786+/-0.291 and 1.442+/-0.175, which were significantly higher than those in adjacent gastric mucosa tissue (0.369+/-0.052 and 0.348+/-0.029) (P<0.01). The expression of NHE1 mRNA was positively correlated with NHE1 protein in the gastric carcinoma tissue (r=0.264, P<0.05). The expressions of NHE1 mRNA and protein were associated with the depth of invasion, lymph node metastasis, and TNM staging (P<0.05). However, no statistical difference was found in age, gender, and tumor differentiation (P>0.05).</p><p><b>CONCLUSION</b>The expression levels of NHE1 mRNA and protein are significantly up-regulated in gastric carcinoma tissue, which may be involved in the development of gastric carcinoma.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Prognosis
/
Stomach
/
Stomach Neoplasms
/
RNA, Messenger
/
Carcinoma
/
Sodium-Hydrogen Exchangers
/
Cation Transport Proteins
/
Sodium-Hydrogen Exchanger 1
/
Metabolism
Type of study:
Prognostic_studies
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Zh
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2010
Document type:
Article