Research on expression of somatomedin b domain of proteoglycan 4 and recombinant protein aggregation / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1319-1324, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-266757
Responsible library:
WPRO
ABSTRACT
Recombinant protein SMB(PRG4) containing two Somatomedin B domains and a small amount of glycosylation of repetitive sequences of proteoglycan 4 was cloned according to PGR4 gene polymorphism. Mature purification process was established and recombinant protein SMB(PRG4), with high-level expression was purified. By using size-exclusion chromatogaraphy and dynamic light scattering, we found that the recombinant protein self-aggregate to dimeric form. Structure prediction and non-reducing electrophoresis revealed that SMB(PRG4), was a non-covalently bonded dimer.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Proteoglycans
/
Glycosylation
/
Recombinant Proteins
/
Somatomedins
/
Chemistry
/
Protein Multimerization
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2014
Document type:
Article