Current Situation of Antimicrobial Resistance and Genetic Differences in Stenotrophomonas maltophilia Complex Isolates by Multilocus Variable Number of Tandem Repeat Analysis / 감염과화학요법
Infection and Chemotherapy
; : 285-293, 2016.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-26690
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Stenotrophomonas maltophilia is one of several opportunistic pathogens of growing significance. Several studies on the molecular epidemiology of S. maltophilia have shown clinical isolates to be genetically diverse. MATERIALS ANDMETHODS:
A total of 121 clinical isolates tentatively identified as S. malophilia from seven tertiary-care hospitals in Korea from 2007 to 2011 were included. Species and groups were identified using partial gyrB gene sequences and antimicrobial susceptibility testing was performed using a broth microdilution method. Multi locus variable number of tandem repeat analysis (MLVA) surveys are used for subtyping.RESULTS:
Based on partial gyrB gene sequences, 118 isolates were identified as belonging to the S. maltophilia complex. For all S. maltophilia isolates, the resistance rates to trimethoprime-sulfamethoxazole (TMP/SMX) and levofloxacin were the highest (both, 30.5%). Resistance rate to ceftazidime was 28.0%. 11.0% and 11.9% of 118 S. maltophilia isolates displayed resistance to piperacillin/tazobactam and tigecycline, respectively. Clade 1 and Clade 2 were definitely distinguished from the data of MLVA with amplification of loci. All 118 isolates were classified into several clusters as its identification.CONCLUSION:
Because of high resistance rates to TMP/SMX and levofloxacin, the clinical laboratory department should consider providing the data about other antimicrobial agents and treatment of S. maltophilia infections with a combination of antimicrobials can be considered in the current practice. The MLVA evaluated in this study provides a fast, portable, relatively low cost genotyping method that can be employed in genotypic linkage or transmission networks comparing to analysis of the gyrB gene.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Ceftazidime
/
Molecular Epidemiology
/
Tandem Repeat Sequences
/
Stenotrophomonas
/
Stenotrophomonas maltophilia
/
Levofloxacin
/
Korea
/
Methods
/
Anti-Infective Agents
Type of study:
Prognostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Infection and Chemotherapy
Year:
2016
Document type:
Article