Qindan capsule changes adventitial collagen synthesis in spontaneously hypertensive rats / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 689-695, 2013.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-267218
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Qindan capsule (QC) on collagen synthesis and the mechanism underlying the process in spontaneously hypertensive rats (SHRs).</p><p><b>METHODS</b>Twentyfour SHRs were divided into three groups the hypertension model group, the QC treatment group, and the losartan treatment group. Eight Wistar Kyoto (WKY) rats were used as the normal control group. The systolic blood pressure (SBP) of the rats was monitored, and the thoracic aorta adventitia of the rats was segregated. The expressions of transforming growth factor 1 (TGF-β1), Smad3, and collagens I and were measured by histological staining and reverse transcription polymerase chain reaction.</p><p><b>RESULTS</b>The SBP was significantly higher in the model group than in the normal control group (P<0.01). However, a significant SBP-lowering effect was observed in QC or losartan treatment groups (P<0.05 or P<0.01) after 3 weeks of treatment. QC-treated rats showed a decrease of approximately 40 mm Hg, and the losartan-treated rats showed a decrease of approximately 50 mm Hg at the end of treatment compared with the beginning of treatment. The protein and gene levels of TGF-β1, Smad3, and collagens I and in the model group were significantly increased compared with those in the normal control group (P<0.01). However, the levels were significantly decreased in the QC or losartan treatment group compared with the model group (P<0.05 or P<0.01). However, there was no significant difference between the QC and losartan treatment groups (P<0.05).</p><p><b>CONCLUSIONS</b>QC could exert its antihypertensive effect through down-regulating TGF-β1-stimulated collagen expressions. The TGF-β1/Smad3 signaling pathway may be involved in this process.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Rats, Inbred SHR
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Rats, Inbred WKY
/
Staining and Labeling
/
Systole
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Blood Pressure
/
Blood Vessels
/
RNA, Messenger
/
Capsules
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2013
Document type:
Article