Analysis of STR loci expression after allogeneic cord blood hematopoietic stem cell transplantation / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 843-846, 2008.
Article
in Zh
| WPRIM
| ID: wpr-267876
Responsible library:
WPRO
ABSTRACT
The purpose of this study was to analyze the STR loci expression after allergenic cord hematopoietic stem cell transplantation in patient with Ducennes muscular dystropy (DMD) patient. PCR-SSO was used to identify the HLA antigens and alleles, STR-PCR was used to detect the chimera status. Quantity analysis of donor chimeras was performed by multiplex PCR amplification of STR marker and capillary electrophoresis with fluorescence detection. The results showed that patient appear to be HLA identical to the donor cord blood at the tested level. Persistent full donor chimerism was found in breast bone marrow. The patient with stable MC (DC < 5%) had a probability of long term survival with molecular remission MC status appeared in forearm muscle, tongue, liver, spleen, stomach, right temporal lobe, diaphragmatic muscle, bronchus, left ventricle and right kidney. In conclusion, the donor gene can express in parenchymatoas organs, the donor chimerism was detected in breast bone marrow and some other organs.
Full text:
1
Database:
WPRIM
Main subject:
Therapeutics
/
Transplantation, Homologous
/
Transplantation Chimera
/
Microsatellite Repeats
/
Cord Blood Stem Cell Transplantation
/
Genetic Loci
/
Genetics
/
Muscular Dystrophies
Type of study:
Prognostic_studies
Limits:
Child
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2008
Document type:
Article