Construction and identification of adenovirus vector for double-mutant human hypoxia-inducible factor-1alpha gene / 南方医科大学学报
Journal of Southern Medical University
; (12): 445-447, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-268110
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct an adenovirus vector containing the double-mutant hypoxia-inducible factor-1alpha (HIF-1alpha) gene for exploring the therapeutic angiogenesis for coronary heart disease.</p><p><b>METHODS</b>Human double-mutant HIF-1alpha cDNA was obtained from PCR of pShuttle-2-HIF-1alpha containing the mutant HIF-1alpha gene (564). The recombinant adenoviral plasmid containing mutant HIF-1alpha cDNA was constructed by ligation of recombinant pShuttle2 containing double-mutant HIF-1alpha cDNA and Adeno-X viral DNA, followed by its identification and transfection into adenoviral packaging cells HEK293 via lipofectamine 2000.</p><p><b>RESULT AND CONCLUSION</b>The recombinant pAdeno-HIF-1alpha was correctly constructed and verified by restriction endonuclease and DNA sequencing analyseis. This recombinant adenovirus containing the double-mutant HIF-1alpha gene may facilitate further investigation of mutant HIF-1alphagene therapy for coronary heart disease.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Therapeutics
/
Genetic Therapy
/
Adenoviridae
/
Coronary Disease
/
Cell Line, Tumor
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
Genetic Vectors
/
Genetics
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Document type:
Article