Alternatively activated macrophages/mononuclear phagocytes promote growth and invasion of breast cancer cell line SKBR3 / 南方医科大学学报
Journal of Southern Medical University
; (12): 410-413, 2007.
Article
in Zh
| WPRIM
| ID: wpr-268120
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of alternatively activated macrophages /mononuclear phagocytes(MNP) on breast cancer cells and explore the mechanisms for the action of tumor-associated macrophages in breast cancer.</p><p><b>METHODS</b>Human peripheral blood monocytes were isolated and cultured in vitro and divided into 3 groups, namely classically activated monocytes (CAM) which were induced by lipopolysaccharide, alternatively activated monocytes (AAM) induce by IL-4, and control cells treated with the culture medium only. After cell culture for 48-72 h, the mRNA of tumor necrosis factor-alpha (TNF-alpha), alternative monocytes activation- associated CC-chemokine 1 (AMAC-1), and beta-actin of the 3 groups were extracted for RT-PCR, or the cells were cocultured with breast cancer cell line SKBR3, or seeded in chicken chorioallantoic membrane along with SKBR3.</p><p><b>RESULTS</b>TNF-alpha mRNA was significantly increased in CAM, and AMAC-1 was highly expressed in AAM. The coculture experiments showed that CAM exhibited obvious inhibitory effect on SKBR3 cells after a 3-day culture whereas AAM significantly promoted the growth of SKBR3 cells after a 5-day culture. In chicken on chorioallantoic membrane experiment, the macrophages promoted tumor angiogenesis and AAM showed the most obvious effect.</p><p><b>CONCLUSION</b>IL-4 induces high expression of AMAC-1, a molecular marker of AAM, in the macrophages, and AAM can promote the growth of SKBR3 cells and tumor angiogenesis.</p>
Full text:
1
Database:
WPRIM
Main subject:
Phagocytes
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Breast Neoplasms
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Interleukin-4
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Tumor Necrosis Factor-alpha
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Coculture Techniques
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Chemokines, CC
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Cell Line, Tumor
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Cell Proliferation
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Allergy and Immunology
/
Macrophage Activation
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2007
Document type:
Article