Effect of FK506 on cytokine secretion in whole blood / 南方医科大学学报
Journal of Southern Medical University
; (12): 249-251, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-269581
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of FK506 on cytokine secretions in whole blood from healthy individuals.</p><p><b>METHODS</b>Blood samples collected from healthy volunteers were co-cultured with different concentrations of FK506 and stimulated with PMA and IONO. The concentrations of 8 cytokines including IL-2, IL-6, IL-12, IL-17, IFN-gamma, TNF-alpha, GM-CSF and G-CSF were detected by Bio-Plex suspension system.</p><p><b>RESULTS</b>Compared with the control group, high-concentration FK506 (20 ng/ml) significantly inhibited the secretions of IL-2, IL-6, IL-12, IL-17, IFN-gamma, TNF-alpha, GM-CSF and G-CSF. At a moderate concentration (5 ng/ml), FK506 inhibited the secretion of GM-CSF significantly.</p><p><b>CONCLUSION</b>FK506 effectively inhibits the secretion of proinflammatory cytokines including IL-6, IFN-gamma and TNF-alpha and also the secretion of IL-2, IL-12, IL-17, GM-CSF and G-CSF. FK506 might play the role of immunosuppression by inhibiting the production of these cytokines by the immune cells. Monitoring the levels of these cytokines might be a potential method for evaluating the adequacy of FK506 doses administered.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Blood
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Down-Regulation
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Cytokines
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Interleukin-6
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Interferon-gamma
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Tumor Necrosis Factor-alpha
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Tacrolimus
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Bodily Secretions
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Immunosuppressive Agents
Limits:
Adult
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Female
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Humans
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Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Document type:
Article