DAPT enhances the apoptosis of human tongue carcinoma cells / 国际口腔科学杂志·英文版
International Journal of Oral Science
; (4): 81-89, 2009.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-269731
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the effect of DAPT (gamma-secretase inhibitor) on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma.</p><p><b>METHODOLOGY</b>Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growth was determined using Indigotic Reduction method. The cell cycle and apoptosis were analyzed by flow cytometry. Real-time PCR and Immuno-Fluorescence (IF) were employed to determine the intracellular expression levels.</p><p><b>RESULTS</b>DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G0-G1 cell cycle arrest and apoptosis. The mRNA levels of Hairy/Enhancer of Split-1 (Hes-1), a target of Notch activation, were reduced by DAPT in a dose-dependent manner. Coincident with this observation, DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells.</p><p><b>CONCLUSION</b>DAPT may have a therapeutic value for human tongue carcinoma. Moreover, the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch-1 and Caspase-3.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Mouth and Oropharynx Cancers
/
Oral Conditions and Disorders
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Repressor Proteins
/
Tongue Neoplasms
/
Carcinoma
/
Cell Membrane
/
Cell Nucleus
/
Resting Phase, Cell Cycle
/
G1 Phase
/
Apoptosis
Limits:
Humans
Language:
English
Journal:
International Journal of Oral Science
Year:
2009
Document type:
Article