The Effect of Adenoviral-mediated Gene Transfer of Bone Morphogenic Protein-7 on Peritoneal Fibrosis in an Animal Model of Peritoneal Dialysis / 대한신장학회지
Korean Journal of Nephrology
; : 421-432, 2008.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-27001
Responsible library:
WPRO
ABSTRACT
PURPOSE:
TGF-beta-induced epithelial-mesenchymal transition (EMT) is associated with peritoneal fibrosis during PD. We conducted this study to evaluate the effect of BMP-7 adenoviral gene transfer on the functional and structural changes of peritoneum and whether it is associated with peritoneal EMT using an animal PD model.METHODS:
Forty Sprague-Dawley rats were divided into five groups; Control (C, n=8), Dialysis (D, n= 8), Rest (R, n=8), BMP-7 (B, n=8) and LacZ (L, n=8) group. Peritoneal function was assessed on baseline, 3rd, 6th, 8th weeks after PD. Immunohistochemistry for TGF-beta, VEGF, laminin and aquaporin-1 was performed in addition to morphometric analysis of peritoneum. Immunofluorescence staining with western blotting for alpha-SMA and E-cadherin, as markers of EMT, was performed.RESULTS:
The thickness of submesothelial matrix was highest in D and significantly decreased in B compared to D, R and L. D/D0 glucose at 8 weeks was significantly increased in B and L compared to that of at 6 weeks, but there were no significant differences among R, B and L at 8 weeks. TGF-beta1 and VEGF expression was observed in submesothelial matrix in D and decreased in R, B and L. Peritoneal fibrosis and functional deterioration of peritoneal membrane were associated with EMT, which was partially reversed in R, B and L.CONCLUSIONS:
BMP-7 gene transfer to peritoneum was not associated with the additive therapeutic effect on peritoneal function compared to the peritoneal rest, although it improved morphologic changes of peritoneum.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Peritoneum
/
Immunohistochemistry
/
Genetic Therapy
/
Cadherins
/
Blotting, Western
/
Transforming Growth Factor beta
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Fluorescent Antibody Technique
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Laminin
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Peritoneal Dialysis
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Rats, Sprague-Dawley
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Nephrology
Year:
2008
Document type:
Article