Direct evidence of the existence of specific LPS binding sites on vascular endothelial cells / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 113-117, 2004.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-270241
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the binding characteristics of endothelial cell (EC) with LPS free from the participation of serum factors.</p><p><b>METHODS</b>Laser confocal microscope was employed in the observation of the binding of EC with FITC-LPS. The KD and the binding sites of each EC were calculated by radioligand binding assay of receptors (RBA) using [(3)H]-LPS.</p><p><b>RESULTS</b>The binding of EC with LPS was saturable, time and concentration dependent and it could be competed with overdosed LPS of the same type. The fluorescence mainly distributed in cytoplasm, especially near the nucleus, which could also be stained.</p><p><b>CONCLUSIONS</b>There might be some specific LPS binding sites existing on ECs and LPS could function intracellularily.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Radioligand Assay
/
Umbilical Veins
/
Cells, Cultured
/
Lipopolysaccharides
/
Fluorescein-5-isothiocyanate
/
Microscopy, Confocal
/
Cell Biology
/
Endothelial Cells
/
Dose-Response Relationship, Drug
/
Fluorescent Dyes
Aspects:
Implementation research
Limits:
Humans
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2004
Document type:
Article