Effects of basic fibroblast growth factor on biological characteristics of osteoblasts / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 229-233, 2003.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-270325
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To elucidate the effects of exogenous basic fibroblast growth factor (bFGF) on biological characteristics of rat osteoblasts cultured in vitro.</p><p><b>METHODS</b>The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-beta(1)) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts.</p><p><b>RESULTS</b>bFGF (5-50 ng/ml) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-beta(1) mRNA increased significantly, but the intracellular ALP content decreased.</p><p><b>CONCLUSIONS</b>bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-beta(1), but cannot promote the differentiation of osteoblasts.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Pharmacology
/
RNA, Messenger
/
Cells, Cultured
/
Fibroblast Growth Factor 2
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Transforming Growth Factor beta
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Rats, Sprague-Dawley
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Proliferating Cell Nuclear Antigen
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Alkaline Phosphatase
/
Transforming Growth Factor beta1
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2003
Document type:
Article