Study on inhibitory effects of c9, t11-conjugated linoleic acid on migration of human gastric carcinoma cell line via cyclooxygenase-2 pathway / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 471-474, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-270476
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the inhibitory effects of c9, t11-conjugated linoleic acid (c9, t11-CLA) on migration of human gastric carcinoma cell line (SGC-7901) via cyclooxygenase-2 (COX-2) pathway.</p><p><b>METHODS</b>After inhibiting COX-2 activity by 100 micromol/L COX-2 inhibitor NS-398 in SGC-7901 cell, we treated SGC-7901 cells with c9, t11-CLA at a concentration of 200,100, 50, 25 micromol/L for 24 h, respectively. Using reconstituted basement membrane invasion, adhesion, chemotaxis assays, we detected the effect of c9, t11-CLA and COX-2 on the cell migration.</p><p><b>RESULTS</b>Compared to NS-398 group, 200, 100 micromol/L c9, t11-CLA significantly suppressed SGC-7901 cells invading into the reconstituted basement membrane (F = 14.309, P = 0.000; F = 19.005, P = 0.000). 200 micromol/L c9, t11-CLA significantly inhibited SGC-7901 cells adhering to laminin, fibronectin and Matrigel (F = 3.063, P = 0.021; F = 6.692, P = 0.001; F = 11.999, P = 0.000). The chemotaxis of SGC-7901 cells and inhibitory frequency were significantly decreased in the 200 micromol/L c9, t11-CLA group (F = 1.380, P = 0.276).</p><p><b>CONCLUSION</b>c9, t11-CLA inhibits invasion, adhesion and chemotaxis of SGC-7901 cells, and the COX-2 plays an important role in the process. [ Key words]</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
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Physiology
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Stomach Neoplasms
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Tumor Cells, Cultured
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Cell Movement
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Linoleic Acid
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Cyclooxygenase 2
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Cyclooxygenase 2 Inhibitors
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Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2007
Document type:
Article