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S phase cell percentage normalized BrdU incorporation rate, a new parameter for determining S arrest / 生物医学与环境科学（英文）
Peng ZHAO; Juan-Ling FU; Bi-Yun YAO; Yong-Rui JIA; Zong-Can ZHOU.
Biomedical and Environmental Sciences ; (12): 215-219, 2014.
Article in English | WPRIM (Western Pacific) | ID: wpr-270611
Responsible library: WPRO
ABSTRACT
In this study, a new parameter, S phase cell percentage (S fraction) normalized BrdU (SFN-BrdU) incorporation rate, was introduced to detect S arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, SFN-BrdU incorporation rate can be used to detecting S arrest.
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Full text: Available Database: WPRIM (Western Pacific) Main subject: DNA Damage / Bromodeoxyuridine / Pharmacokinetics / S Phase / Cell Biology / Cell Proliferation / Epithelial Cells / S Phase Cell Cycle Checkpoints Limits: Humans Language: English Journal: Biomedical and Environmental Sciences Year: 2014 Document type: Article
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Full text: Available Database: WPRIM (Western Pacific) Main subject: DNA Damage / Bromodeoxyuridine / Pharmacokinetics / S Phase / Cell Biology / Cell Proliferation / Epithelial Cells / S Phase Cell Cycle Checkpoints Limits: Humans Language: English Journal: Biomedical and Environmental Sciences Year: 2014 Document type: Article