Rapid purification of human albumin with immunomagnetic microsphere technology / 药学学报
Acta Pharmaceutica Sinica
; (12): 608-614, 2006.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-271399
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To develop a rapid and inexpensive method for purification of human albumin, a method of immunomagnetic microspheres (IMMS) based on enzyme-linked immunosorbent assay (ELISA) for the purification of human albumin from human serum.</p><p><b>METHODS</b>Polystyrene magnetic microspheres with carboxyl groups as carriers were prepared, and then the carboxyl groups on the surface of the microspheres were activated by ethylcarbodiimide (EDC). Finally rabbit anti-human serum albumin (HSA) antibodies were covalently bound to it and the complex can specifically capture HSA. After the procedure of capturing HSA, through taking rabbit anti-human albumin protein antibodies as a capture antibody, and goat anti-human albumin protein antibodies as a detection antibody, an ELISA on IMMS was developed, which can determine the recovery yield of HSA from the human serum.</p><p><b>RESULTS</b>The result of the experiment was that the recovery of human albumin with IMMS was (86 +/- 4)%, and IMMS were reused for two other purifying cycles, the results of which were (69.0 +/- 0.6)% and (40.8 +/- 0.8)%, and the purity of the product was about 90%.</p><p><b>CONCLUSION</b>The results above prove that the immunomagnetic purifiying strategy was shown to be efficient and offers an new thought for a large scale production of high-purity HSA.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Polystyrenes
/
Serum Albumin
/
Enzyme-Linked Immunosorbent Assay
/
Chemistry
/
Reproducibility of Results
/
Immunomagnetic Separation
/
Allergy and Immunology
/
Methods
/
Microspheres
Limits:
Humans
Language:
English
Journal:
Acta Pharmaceutica Sinica
Year:
2006
Document type:
Article