Detection of ROS1 gene rearrangement by FISH and analysis of its clinical features in non-small cell lung cancer patients / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 751-754, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-272298
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the frequency of ROS1 gene rearrangement in non-small cell lung cancer ( NSCLC) patients by FISH, and to analyze the relationship between ROS1 gene rearrangement and clinical features (including age, sex, stage, histology, smoking history) with NSCLC.</p><p><b>METHODS</b>The ROS1 gene rearrangement in histological sections of 1 652 NSCLC tissues was detected by FISH. The extracted RNA was amplified and the sequences were analyzed by Sanger sequencing for ROS1-positive samples.</p><p><b>RESULTS</b>ROS1 rearrangement was identified in 53 specimens (3.2%) from the 1 652 NSCLC tissues. Among these positive cases, 15 were CD74-ROS1, 13 were SLC34A2-ROS1, 13 were SDC4-ROS1 and 12 were TPM3-ROS1. The frequency of ROS1 rearrangement was significantly higher in never-smoking patients (49 cases) than in smokers (4 cases) (P < 0.05). Patients with ROS1-positive NSCLC tended to be younger and there was no significant difference in sex (P > 0.05). All of the ROS1-positive samples were adenocarcinomas, with a tendency toward higher clinical stage (P < 0.05).</p><p><b>CONCLUSIONS</b>ROS1 rearrangement has diversity, and may be defined as a new molecular subtype of NSCLC. ROS1 rearrangement tends to occur in younger, and never-smoker lung adenocarcinoma patients.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Oncogenes
/
Protein-Tyrosine Kinases
/
Gene Rearrangement
/
Adenocarcinoma
/
Proto-Oncogene Proteins
/
In Situ Hybridization, Fluorescence
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Carcinoma, Non-Small-Cell Lung
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Genetics
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Lung Neoplasms
/
Metabolism
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2014
Document type:
Article