Protective effect of dexmedetomidine against glutamate-induced cytotoxicity in PC12 cells and its mechanism / 南方医科大学学报
Journal of Southern Medical University
; (12): 150-156, 2016.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-273795
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of dexmedetomidine (Dex) against glutamate-induced cytotoxicity in PC12 cells and its mechanism.</p><p><b>METHODS</b>PC12 cells were treated with varying concentrations of dexmedetomidine 1 h before exposure to a high concentration of glutamate. The cell viability was measured by MTT assay, and LDH release, MDA content and SOD activity were measured. The level of ROS was tested by DCFH-DA staining and flow cytometry. The level of intracellular Cawas detected by Fluo-8 staining and flow cytometry, and the mitochondrial membrane potential (MMP) was determined with JC-1 staining and flow cytometry.</p><p><b>RESULTS</b>Within the concentration range of 0.01 to 100 µmol/L, Dex dose-dependently protected PC12 cells against glutamate-induced cytotoxicity. Treatment with 100 µmol/L Dex significantly increased the cell viability to (86.6∓2.2)% of that of the control cells (P<0.01) and decreased LDH release to 1.4∓0.1 folds of the control level (P<0.01). In PC12 cells exposed to glutamate, Dex pretreatment significantly reduced MDA content (P<0.01), enhanced SOD activity (P<0.01), inhibited ROS overproduction (P<0.01), reduced intracellular Calevel (P<0.01) and maintained a stable MMP (P<0.01).</p><p><b>CONCLUSION</b>Dexmedetomidine can protect PC12 cells against glutamate-induced injury possibly in relation with its anti-oxidative activity, inhibitory effect on intracellular calcium overload and protective effect of the mitochondria.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cell Survival
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Calcium
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PC12 Cells
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Reactive Oxygen Species
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Apoptosis
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Glutamic Acid
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Dexmedetomidine
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Membrane Potential, Mitochondrial
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Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2016
Document type:
Article