Establishment of Integration-Free Human Induced Pluripotent Stem Cells from A Patient with Primary Myelofibrosis / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1415-1421, 2015.
Article
in Zh
| WPRIM
| ID: wpr-274024
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the primary myelofibrosis (PMF)-induced pluripotent stem cell line (iPSC) by means of iPSC techinique so as to provide a experimental model for studying the blood disease mechanisms.</p><p><b>METHODS</b>Induced pluripotent stem cells were generated from mononuclear cells isolated from a PMF patient with JAK2(V617F) mutation by using episomal vectors.</p><p><b>RESULTS</b>PMF-derived iPSC was established from the patient with JAK2(V617F) gene mutation. The PMF-iPSC could be stably passaged, highly expressed pluripotent genes as human embryonic stem (ES) cells, and were able to form teratoma in NOD/SCID mice in vivo. H & E staining of the teratoma showed the presence of tissue type derived from all three embryonic germ layers. Sanger sequencing confirmed that PMF-derived iPSC carried different allele burdens of JAK2(V617F) gene mutation.</p><p><b>CONCLUSION</b>The interation-free iPSC from primary myelofibrosis patient in vitro has been established. This PMF-derived iPSC line provides a valuable tool for studying the pathogenesis, screening of chimical drugs and realizing the standard therapy of PMF.</p>
Full text:
1
Database:
WPRIM
Main subject:
Mice, SCID
/
Mice, Inbred NOD
/
Cell Culture Techniques
/
Alleles
/
Janus Kinase 2
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Primary Myelofibrosis
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Induced Pluripotent Stem Cells
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Genetics
/
Mutation
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2015
Document type:
Article