Study on andrographolide-induced apoptosis of Candida albicans biofilm dispersion cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 362-365, 2012.
Article
in Zh
| WPRIM
| ID: wpr-274342
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the effect of andrographolide on apoptosis of Candida albicans biofilm dispersion cells.</p><p><b>METHOD</b>The morphological changes of apoptotic C. albicans biofilm cells were observed by using Hoechst 33258 staining Fluorescence microscope; changes of mitochondrial membrane potential (MMP) of C. albicans biofilm cells were detected by rhodamine 123 staining flow cytometry; and reactive oxygen species (ROS) was detected by DHR staining flow cytometry.</p><p><b>RESULT</b>1 000, 100 micromol x L(-1) of andrographolide could cause pyknosis and dense staining of C. albicans biofilm cells, 1 000, 100, 10 micromol x L(-1) of andrographolide could decrease MMP and increase ROS of C. albicans biofilm cells.</p><p><b>CONCLUSION</b>Andrographolide of appropriate concentrations could induce apoptosis of dispersion cells of C. albicans biofilms.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Physiology
/
Candida albicans
/
Reactive Oxygen Species
/
Apoptosis
/
Biofilms
/
Diterpenes
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Membrane Potential, Mitochondrial
/
Metabolism
/
Antifungal Agents
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Document type:
Article