DNA Vaccines Encoding Toxoplasma gondii Cathepsin C 1 Induce Protection against Toxoplasmosis in Mice
The Korean Journal of Parasitology
; : 505-512, 2017.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-27500
Responsible library:
WPRO
ABSTRACT
Toxoplasma gondii cathepsin C proteases (TgCPC1, 2, and 3) are important for the growth and survival of T. gondii. In the present study, B-cell and T-cell epitopes of TgCPC1 were predicted using DNAstar and the Immune Epitope Database. A TgCPC1 DNA vaccine was constructed, and its ability to induce protective immune responses against toxoplasmosis in BALB/c mice was evaluated in the presence or absence of the adjuvant α-GalCer. As results, TgCPC1 DNA vaccine with or without adjuvant α-GalCer showed higher levels of IgG and IgG2a in the serum, as well as IL-2 and IFN-γ in the spleen compared to controls (PBS, pEGFP-C1, and α-Galcer). Upon challenge infection with tachyzoites of T. gondii (RH), pCPC1/α-Galcer immunized mice showed the longest survival among all the groups. Mice vaccinated with DNA vaccine without adjuvant (pCPC1) showed better protective immunity compared to other controls (PBS, pEGFP-C1, and α-Galcer). These results indicate that a DNA vaccine encoding TgCPC1 is a potential vaccine candidate against toxoplasmosis.
Full text:
Available
Health context:
Sustainable Health Agenda for the Americas
/
SDG3 - Health and Well-Being
/
Neglected Diseases
Health problem:
Goal 10: Communicable diseases
/
Target 3.3: End transmission of communicable diseases
/
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Peptide Hydrolases
/
Spleen
/
Toxoplasma
/
DNA
/
Immunoglobulin G
/
B-Lymphocytes
/
Cathepsins
/
Toxoplasmosis
/
Interleukin-2
/
Epitopes, T-Lymphocyte
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2017
Document type:
Article