Pre-L-methionine feeding strategy for S-adenosyl-L-methionine fermentative production / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1824-1827, 2008.
Article
in Zh
| WPRIM
| ID: wpr-275333
Responsible library:
WPRO
ABSTRACT
The yield of S-adenosyl-L-methionine (SAM) on high-cell-density fermentation by saccharomyces cerevisiae is mostly affected by the feeding strategy of pre-L-methionine. The mutant strain SAM0801 that could accumulate more SAM was used in this study. Six high-cell-density fermentation experiments in 5 L fermentor were investigated to get the optimal feeding time and amount of L-methionine. The results showed that when 40 g L-methionine was added in the fermentor after 30 h fermentation, a dry cell weight of 100 g/L was achieved. Under this condition, after 58 h fermentation, both the dry cell weight and the yield of SAM reached the maximum, 168 g/L and 14.48 g/L respectively.
Full text:
1
Database:
WPRIM
Main subject:
S-Adenosylmethionine
/
Saccharomyces cerevisiae
/
Bioreactors
/
Fermentation
/
Genetics
/
Metabolism
/
Methionine
/
Microbiology
/
Mutation
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2008
Document type:
Article