N01WA-735E, a human leukocyte elastase inhibitor from metabolites of microorganisms / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1112-1115, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-276152
Responsible library:
WPRO
ABSTRACT
Human leukocyte elastase is an important selection target of inflammation and cancer. In this paper, a high throughput screening model was established for screening human leukocyte elastase inhibitors from thousands of strains of actinomycetes. As a result, a strain, N01WA-735 with potent suppression activity was isolated. Firstly, the strain N01WA-735 was identified as Streptomyces according to morphology and biochemical analysis. The Streptomyces N01WA-735 was processed by solvent extraction, silica column chromatography, Sephadex LH-20 column chromatography and crystallization to get a pure active compound named N01WA-735E. Its chemical structure was elucidated as the same as that of the compound named BE-52440A by physicochemical properties and spectral data of UV, MS, 1H-NMR and 13C-NMR respectively. The compound showed a strong inhibitory activity against human leukocyte elastase with IC50 of 3.02 micromol/L. The compound is reported as a human leukocyte elastase inhibitor for the first time.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Protease Inhibitors
/
Streptomyces
/
Leukocyte Elastase
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Document type:
Article