Study of target pegylated recombinant mutant human granulocyte colony stimulating factor / 生物工程学报
Chinese Journal of Biotechnology
; (12): 919-923, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-276186
Responsible library:
WPRO
ABSTRACT
Recombinant mutant human granulocyte colony stimulating factor (rmhG-CSF) was pegylated, purified and characterized. rhG-CSF was mutated in position 1,3,4,5,17, and cysteine was added in C-terminal. rmhG-CSF was pegylated by PEG-Mal 20000 and separated by ion-exchange chromatography, gel filtration chromatography. Analysis of SDS-PAGE showed thar the purity of the separated PEG-rmhG-CSF was greater than 95%. and in intro and in vivo bioactivity study showed that target modified PEG-rmhG-CSF kept full bioactivity which was better than traditional pegylation method, and longer half-life was proved in mice.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Polyethylene Glycols
/
Recombinant Proteins
/
Protein Sorting Signals
/
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
Granulocyte Colony-Stimulating Factor
/
Chromatography, Ion Exchange
/
Amino Acid Sequence
/
Mutant Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Document type:
Article