Induction of anti-lymphoma cytotoxic T cell effect by dendritic cells transfected with recombinant adenovirus vectors carrying survivin gene / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 591-593, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-276866
Responsible library:
WPRO
ABSTRACT
This study was purposed to investigate the immunological effects of modified dendritic cells (DCs) in inducing cytotoxic T cells (CTLs) effect against lymphoma cells. The DCs were derived from human peripheral blood and transfected with recombined adenovirus vector carrying survivin gene, Western blot was used to detect the expression of survivin, the lactate dehydrogenase (LDH) release test was used to determine the cytotoxicity of CTLs, the mixed lymphocyte reaction (MLR) was used to measure the ability to proleferate allo-lymphocyte by DCs, ELISA was used to assay IL-12 level in supernatant. The results showed that the expression of survivin in transfected dentritic cells was confirmed by Western blot analysis. In MLR assay, DCs transfected with Ad-survivin could induce higher allogeneic lymphocytes reaction at the ratios of 15, 110, 150 and 1100. DCs transfected with Ad-survivin had much higher activity of CTL to CA46 cells than control DCs. The levels of IL-12 of supernatants containing DCs transfected with Ad-survivin were significantly higher than that in the control group. It is concluded that DCs transfected with Ad-survivin can induce CTL response in vitro against lymphoma cells.
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Lymphomas and Multiple Myeloma
Database:
WPRIM (Western Pacific)
Main subject:
Recombination, Genetic
/
Dendritic Cells
/
Tumor Cells, Cultured
/
Transfection
/
T-Lymphocytes, Cytotoxic
/
Adenoviridae
/
Apoptosis
/
Interleukin-12
/
Allergy and Immunology
/
Inhibitor of Apoptosis Proteins
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2007
Document type:
Article