Determination of betamethasone in human plasma by liquid chromatography with tandem mass / 药学学报
Acta Pharmaceutica Sinica
; (12): 402-407, 2008.
Article
in En
| WPRIM
| ID: wpr-277840
Responsible library:
WPRO
ABSTRACT
A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of betamethasone in human plasma. The analyte was isocratically eluted on a Venusil XBP C8 column (200 mm x 3.9 mm ID, 5 microm) with methanol-water mol x L(-1) ammonium formate) (80:20) at a flow rate of 0.4 mL x min(-1), and detected (containing 5 mmol x L(-1) ammonium formate) (80:20) at a flow rate of 0.4 mL x min(-1), and detected with a triple quad LC-MS/MS using ESI with positive ionization. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 393.3-->355.2 for betamethasone and m/z 361.3-->343.2 for prednisolone (IS). Betamethasone was extracted from 0.5 mL human plasma with ethyl acetate. The average recovery is 88.24% and the low limit of quantitation (LLOQ) was 0.5 ng x mL(-1). The 3-day validation study demonstrated excellent precision and accuracy across the calibration range of 0.5-80.0 ng x mL(-1). The method was successfully applied to the pharmacokinetic study of compound betamethason injection in healthy Chinese volunteers.
Full text:
1
Database:
WPRIM
Main subject:
Blood
/
Betamethasone
/
Pharmacokinetics
/
Chromatography, Liquid
/
Area Under Curve
/
Spectrometry, Mass, Electrospray Ionization
/
Tandem Mass Spectrometry
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Injections, Intramuscular
/
Methods
/
Anti-Inflammatory Agents
Type of study:
Prognostic_studies
Limits:
Humans
/
Male
Language:
En
Journal:
Acta Pharmaceutica Sinica
Year:
2008
Document type:
Article