Content determination of dencichine in Panax Notoginseng by a reversed phase ion-pair chromatography / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4026-4030, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-279290
Responsible library:
WPRO
ABSTRACT
To build a reversed phase ion-pair chromatography to determination content of Dencichine from Panax notoginseng. Using Tetrabutyl ammonium hydroxide ions by the combination of reagent and HPLC method without derivatization to test the content of dencichine directly. The optimum conditions of supersonic extraction were solid-to-liquid ratio 1 20, Continuous ultrasonic extraction twice, each time 15 minutes; 3,500 r · min⁻¹, then centrifuging 15 minutes. Dencichine in different age, place, part and the different Processing mode were examined. The method is simple with sound separation degree and stability, which can facilitate the determination of dencichine content directly and provide the basis in quality standard of raw material.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Chemistry
/
Plant Roots
/
Panax notoginseng
/
Chromatography, Reverse-Phase
/
Amino Acids, Diamino
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Document type:
Article