Effect of urotensin II on the nitric oxide production in neonatal rat cardiomyocytes / 生理学报
Acta Physiologica Sinica
; (6): 307-310, 2002.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-279293
Responsible library:
WPRO
ABSTRACT
The aim of this study was to investigate the effect of urotensin II (U II) on the nitric oxide (NO) production in cultured neonatal rat cardiomyocytes. The endothelial nitric oxide synthase (eNOS) mRNA expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction. The activity of nitric oxide synthase (NOS) and NO content in cardiomyocytes were measured. The current results showed that U inhibited eNOS mRNA expression, the NOS activity and the NO production of cardiomyocytes. U II (0.1 micromol/L) inhibited the NOS activity and the NO production in cardiomyocytes in a time-dependent manner. These results suggest that the cardiovascular effect of U II might be partially associated with NO production in cultured neonatal rat cardiomyocytes.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Urotensins
/
Cells, Cultured
/
Myocytes, Cardiac
/
Nitric Oxide Synthase Type III
/
Animals, Newborn
/
Metabolism
/
Nitric Oxide
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2002
Document type:
Article