Study on HPLC fingerprinting and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2829-2833, 2010.
Article
in Zh
| WPRIM
| ID: wpr-279345
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the HPLC fingerprint and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.</p><p><b>METHOD</b>HPLC analysis was performed on a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) with 0.04 mol x L(-1) ammonium acetate (adjusted to pH 10.0 with ammonia water)and acetonitrile as mobile phase. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004 AB)was used in data analysis.</p><p><b>RESULT</b>Baifupian, Heifupian from different samples were of high similarity in fingerprint, and the separation of six kinds of ester-type alkaloids was good. Huangfupian and crude aconite root showed significant difference in fingerprint, comparing with Baifupian and Heifupian.</p><p><b>CONCLUSION</b>This method is simple and reliable. The HPLC fingerprint and contents of six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice can be used for their quality control.</p>
Full text:
1
Database:
WPRIM
Main subject:
Quality Control
/
Reference Standards
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Drugs, Chinese Herbal
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Chemistry
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Chromatography, High Pressure Liquid
/
Aconitum
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Alkaloids
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Medicine, Chinese Traditional
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Methods
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Document type:
Article