Comparison of rutin and syringin content between tissue culturing seedlings and botanical drug of Saussurea involucrata / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2666-2668, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-279379
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare the rutin and syringin content in tissue culturing seedlings and in botanical drug of Saussurea involucrata.</p><p><b>METHOD</b>The HPLC with Hydro-RP C18 (4.6 mm x 250 mm, 5 microm) column was used, a mixture of acetonitrile-water (595) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 25 degrees C and detection wavelength at 220 nm.</p><p><b>RESULT</b>The effective constituents of tissue culturing seedlings were almost similar to the botanical drug. And syringin in tissue culturing seedlings was increased 4.35 times.</p><p><b>CONCLUSION</b>It has a good prospect to acquire high-quality S. involucrata by tissue culturing seedlings.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phenylpropionates
/
Rutin
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Saussurea
/
Seedlings
/
Glucosides
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Document type:
Article