Metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2585-2589, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-279395
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide.</p><p><b>METHOD</b>Gossypetin-8-O-beta-D-glucuronide was administrated orally at the dose of 250 mg x kg(-1) to healthy rats, then the urine was collected, pre-treated appropriately and detected by HPLC-DAD-MS(n).</p><p><b>RESULT</b>In the rat urine, gossypetin-8-O-beta-D-glucuronide, along with 6 metabolites, were detected, and their structures were elucidated on the basis of reference standards, UV and MS(n) spectra. The metabolic pathways of gossypetin-8-O-beta-D-glucuronide included mainly deglycosylation, glucuronidation and methylation.</p><p><b>CONCLUSION</b>A rapid and sensitive method for the analysis of metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide was established and this will be useful for the further metabolism study of gossypetin-8-O-beta-D-glucuronide.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Flavonoids
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Magnetic Resonance Spectroscopy
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Administration, Oral
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Chromatography, High Pressure Liquid
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Rats, Sprague-Dawley
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Metabolism
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Document type:
Article