Analyses of Binding Profiles of the GII. 12 Norovirus with Human Histo-blood Group Antigens / 病毒学报
Chinese Journal of Virology
; (6): 164-169, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-280279
Responsible library:
WPRO
ABSTRACT
Interactions between noroviruses (NoVs) and the receptors of histo-blood group antigens (HB-GAs) affect the infectivity and host susceptibility of NoVs. We elucidated the binding profile of a GII. 12 NoV to HBGAs. First, we synthesized the P domain sequence of the GII. 12 NoV strain Pune (GenBank accession number EU921353). Protein of the P domain was expressed in a prokaryotic system. Formation of the P particle was monitored by gel-filtration chromatography. Antiserum was prepared by immunization of mice with GII. 12 P particles. The binding profile of the GII. 12 NoV Pune strain was determined by binding of the P particle with a panel of saliva samples with various known HBGAs phenotypes. The GII. 12 NoV was bound strongly to saliva samples of subjects with B and AB types and weakly to A, O secretor, and non-secretor saliva samples, suggesting higher affinity with B antigen by GII. 12 NoV. These results were consistent with those determined by a previous crystallography study of GII. 12 NoV. These data suggested that individuals with B and AB blood types may be more susceptible to infection by GII. 12 NoV compared with those with other blood types. Our findings may provide a basis for the prevention and control of an epidemic of GII. 12 NoV.
Full text:
Available
Health context:
SDG3 - Health and Well-Being
Health problem:
Target 3.3: End transmission of communicable diseases
Database:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Receptors, Virus
/
Viral Proteins
/
Virology
/
Blood Group Antigens
/
Caliciviridae Infections
/
Norovirus
/
Gastroenteritis
/
Genetics
/
Genotype
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2015
Document type:
Article