Prophylactic protective effects of paeoniflorin on corticosterone-induced primary cultures of rat cortical neurons damages / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 208-210, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-281050
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the prophylactic neuroprotective effect of paeoniflorin (PF) on corticosterone-induced primary cultures of rat cortical neurons damages.</p><p><b>METHOD</b>Primary cultures of neurons from the of 16-days-old embryo rats were used to study. Neurons in the experimental group were treated with cort (200 micromol x L(-1)) 30 min after the pretreatment of PF (0.5, 2, 10 micromol x L(-1)). We used MTT assay to detect the action of paeoniflorin on neuronal activity. DAPI staining to study of the influence of PF on neuronal apoptosis morphology, and Annexin V-FITC/PI apoptosis detection kit to inspect the impact of paeoniflorin on the early neuronal apoptosis.</p><p><b>RESULT</b>Compared with model group, every dose of PF (0.5, 2, 10 micromol x L(-1)) can raise the neuronal activity notably (P<0.05), PF of 2 micromol x L(-1) and 10 micromol x L(-1) can reduce the rate of neuronal apoptosis.</p><p><b>CONCLUSION</b>Our data indicated that PF achieved prophylactic neuroprotective effect through against corticosterone-induced neurons damages.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Benzoates
/
Bridged-Ring Compounds
/
Corticosterone
/
Cells, Cultured
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Cerebral Cortex
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Rats, Sprague-Dawley
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Apoptosis
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Cell Biology
/
Monoterpenes
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Document type:
Article