Comparison of apoptosis of articular chondrocytes in the pathogenesis of Kashin-beck disease and primary osteoarthritis / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 267-270, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-281218
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate chondrocyte apoptosis and expression of Fas and inducible nitric oxide synthase (iNOS) in articular cartilage in the pathogenesis of Kashin-beck disease (KBD) and primary osteoarthritis (OA).</p><p><b>METHODS</b>The collected samples of articular cartilage were divided into three groups normal control (15 cases), KBD adults (15 cases) and OA (15 cases). Chondrocyte apoptosis was detected by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling method, and Fas and iNOS in articular cartilage were stained by immunohistochemistry.</p><p><b>RESULTS</b>The positive percentages of chondrocyte apoptosis stained in articular cartilage of KBD and OA were significantly higher than that of the control (P < 0.01), and the positive percentage of chondrocytes apoptosis in the eroded areas of articular cartilage were significantly higher than in the non-eroded areas in articular cartilage of the same patient with KBD and OA (P < 0.05). There was no significant difference in positive percentage of chondrocytes apoptosis between KBD and OA. The positive percentages of Fas and iNOS in chondrocytes were significantly higher in KBD and OA than in control (P < 0.01). Significant differences in Fas and iNOS expression between the eroded areas and non-eroded areas were seen in articular cartilage of patients with KBD and OA (P < 0.05), but such difference did not exist between KBD and OA.</p><p><b>CONCLUSION</b>Cell apoptosis seems to be associated with the pathogenesis of both KBD and OA. Fas and iNOS might mediate chondrocyte apoptosis.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Musculoskeletal Diseases and Rheumatic Disorders
Database:
WPRIM (Western Pacific)
Main subject:
Osteoarthritis
/
Pathology
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Cartilage, Articular
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Apoptosis
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Nitric Oxide Synthase
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Fas Receptor
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Endemic Diseases
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Chondrocytes
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Osteoarthritis, Knee
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In Situ Nick-End Labeling
Type of study:
Etiology study
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2006
Document type:
Article