Dystrophin expression in mdx mice after bone marrow stem cells transplantation / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 178-181, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-281237
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the dynamic changes of dystrophin expression in mdx mice after bone marrow stem cells transplantation.</p><p><b>METHODS</b>The bone marrow stem cells of C57 BL/6 mice (aged 6 to 8 weeks) were injected intravenously into the mdx mice (aged 7 to 9 weeks), which were preconditioned with 7Gy gamma ray. The amount of dystrophin;expression in gastrocnemius was detected by immunofluorescence, reverse transcription-polymerase chain reaction and Western blot at week 5, 8, 12 and 16 after transplantation.</p><p><b>RESULTS</b>At week 5 after bone marrow stem cells transplantation, the dystrophin expression detected in mdx mice were very low; however, its expression increased along with time. At week 16 week, about 12% muscle cells of all transplanted mice expressed dystrophin. There were less centrally placed myonuclei than the control mdx mice, whereas peripheral myonuclei increased.</p><p><b>CONCLUSIONS</b>After having been injected into mdx mice, the allogenic bone marrow stem cells have a trend to reach the injured muscle tissues and differentiate to fibers that can express dystrophin and the expression increased with time. The bone marrow stem cells participates in the repair and regeneration of the injured tissues permanently and constantly.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
General Surgery
/
Transplantation, Homologous
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Bone Marrow Cells
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Cell Differentiation
/
Dystrophin
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Mice, Inbred mdx
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Hematopoietic Stem Cell Transplantation
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Muscular Dystrophy, Duchenne
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Cell Biology
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Disease Models, Animal
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2006
Document type:
Article