Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 729-731, 2010.
Article
in Zh
| WPRIM
| ID: wpr-281728
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata.</p><p><b>METHOD</b>The analysis was carried out on a Boston Crest ODS column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water as mobile phases. The flow rate was 1.0 mL x min(-1) and the detection wavelength was at 215 nm.</p><p><b>RESULT</b>The calibration curve was linear over the range of 0.0202-20.02 microg for kirenol. The correlation coefficient of the calibration curve was 0.99975. The average recovery was 99.62% with relative standard derivation (RSD) of 2.0%.</p><p><b>CONCLUSION</b>The results showed that the method is simple, accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.</p>
Full text:
1
Database:
WPRIM
Main subject:
Acetonitriles
/
Quality Control
/
Sweetening Agents
/
Drugs, Chinese Herbal
/
Carcinogens
/
Adenosine
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Chemistry
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Chromatography, High Pressure Liquid
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Asteraceae
/
Methods
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Document type:
Article