Effects of conjugated linoleic acid on the expression of critical enzymes of linoleic acid metabolism in tumor cell / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 195-198, 2005.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-282361
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To study the effects of c9,t11-conjugated linoleic acid (c9,t11-CLA) on critical enzymes of linoleic acid metabolism in stomach granular cell (SGC-7901).</p><p><b>METHODS</b>SGC-7901 was treated with c9,t11-CLA by 200, 100, 50 or 25 micromol/L for 24 hours. The effects of c9,t11-CLA on the cell proliferation was measured by monotetrazolium and the expression of Delta6-desaturase, Delta5-desaturase, COX-1, COX-2, 5-LOX mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>At a concentration of 200, 100, 50, or 25 micromol/L, c9,t11-CLA suppressed the proliferation of SGC-7901 by 54.3%, 20.5%, 10.5% and 2.93%. The c9,t11-CLA might decrease the expression of COX-2 mRNA, and increase the expression of Delta6-desaturase and COX-1 in SGC-7901, but might not affect Delta5-desaturase and 5-LOX.</p><p><b>CONCLUSION</b>The effects of c9,t11-CLA on the COX and Delta6-desaturase might play an important role in mediating the ability of c9,t11-CLA as to inhibiting the proliferation of tumor cells, and the anti-cancer activity by c9,t11-CLA might be associated with the linoleic acid metabolism.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Linoleic Acids
/
Gene Expression Regulation, Enzymologic
/
Gene Expression Regulation, Neoplastic
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Lipoxygenase
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Reverse Transcriptase Polymerase Chain Reaction
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Gene Expression Profiling
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Cell Line, Tumor
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Linoleic Acids, Conjugated
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2005
Document type:
Article